An AS-PCR assay for accurate genotyping of FAD2A/FAD2B genes in peanuts (Arachis hypogaea L.)

• Autores: H. T. Yu, W. Q. Yang, Y. Y. Tang, X. Z. Wang, Q. Wu
• Localización: Grasas y aceites, ISSN-e 1988-4214, ISSN 0017-3495, Vol. 64, Nº 4, 2013, págs. 395-399
• Idioma: inglés
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• Resumen

  • FAD2A and FAD2B are homoeologous genes from A and B genomes in cultivated peanuts (Arachis hypogaea L.) encoding fatty acid desaturates which convert oleate to linoleate. To study the genetics of oleate and breed high oleate peanut cultivars, a simple allele specific-PCR (AS-PCR) protocol for the accurate genotyping of FAD2A/FAD2B was developed to discriminate the wild and mutant allele of both genes (FAD2A 448 G > A and FAD2B 441_442insA). The results may serve to develop a feasible procedure for producing highly desired high oleate peanut cultivars through hybridization.