Gene methylation parallelisms between peripheral blood cells and oral mucosa samples in relation to overweight

R. San Cristobal; Santiago Navas Carretero; Fermín Ignacio Milagro Yoldi; José Ignacio Riezu Boj; Elizabeth Guruceaga; Carlos Celis Morales; Katherine M. Livingstone; L. Brennan; Julie A. Lovegrove; Hannelore Daniel; Wim H.M. Saris; Iwonna Traczyk; Yannis Manios; Eileen R. Gibney; Michael J. Gibney; J.C. Mathers; José Alfredo Martínez Hernández

Ayuda

Gene methylation parallelisms between peripheral blood cells and oral mucosa samples in relation to overweight

Rodrigo San-Cristobal ^[1] ; Santiago Navas-Carretero ^[1] ; Fermín I. Milagro ^[1] ; J. Ignacio Riezu-Boj ^[1] ; Elizabeth Guruceaga ^[9] ; Carlos Celis-Morales ^[2] ; Katherine M. Livingstone ^[2] ; Lorraine Brennan ^[3] ; Julie A. Lovegrove ^[7] ; Hannelore Daniel ^[8] ; Wim H. Saris ^[4] ; Iwonna Traczyk ^[5] ; Yannis Manios ^[6] ; Eileen R. Gibney ^[3] ; Michael J. Gibney ^[3] ; John C. Mathers ^[2] ; J. Alfredo Martinez ^[1]
1. [1] Universidad de Navarra
  
  Universidad de Navarra
  
  Pamplona, España
2. [2] Newcastle University
  
  Newcastle University
  
  Reino Unido
3. [3] University College Dublin
  
  University College Dublin
  
  Irlanda
4. [4] Maastricht University Medical Centre
  
  Maastricht University Medical Centre
  
  Países Bajos
5. [5] Medical University of Warsaw
  
  Medical University of Warsaw
  
  Warszawa, Polonia
6. [6] Harokopio University
  
  Harokopio University
  
  Dimos Athens, Grecia
7. [7] University of Reading
  
  University of Reading
  
  Reino Unido
8. [8] Technical University Munich
  
  Technical University Munich
  
  Kreisfreie Stadt München, Alemania
9. [9] Instituto de Investigación Sanitaria de Navarra, España
Mostrar afiliaciones +
Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 73, Nº. 3 (August 2017), 2017 (Ejemplar dedicado a: CTPIOD meeting (Contribution To Progress in Obesity and Diabetes Research) 2016), págs. 465-474
Idioma: inglés
Enlaces
- Texto completo (pdf)
Resumen
- Epigenetics has an important role in the regulation of metabolic adaptation to environmental modifications. In this sense, the determination of epigenetic changes in non-invasive samples during the development of metabolic diseases could play an important role in the procedures in primary healthcare practice. To help translate the knowledge of epigenetics to public health practice, the present study aims to explore the parallelism of methylation levels between white blood cells and buccal samples in relation to obesity and associated disorders. Blood and buccal swap samples were collected from a subsample of the Spanish cohort of the Food4Me study. Infinium HumanMethylation450 DNA Analysis was carried out for the determination of methylation levels. Standard deviation for β values method and concordance correlation analysis were used to select those CpG which showed best parallelism between samples. A total of 277 CpGs met the criteria and were selected for an enrichment analysis and a correlation analysis with anthropometrical and clinical parameters. From those selected CpGs, four presented high associations with BMI (cg01055691 in GAP43; r = −0.92 and rho = −0.84 for blood; r = −0.89 and rho = −0.83 for buccal sample), HOMA-IR (cg00095677 in ATP2A3; r = 0.82 and rho = −0.84 for blood; r = −0.8 and rho = −0.83 for buccal sample) and leptin (cg14464133 in ADARB2; r = −0.9182 and rho = −0.94 for blood; r = −0.893 and rho = −0.79 for buccal sample). These findings demonstrate the potential application of non-invasive buccal samples in the identification of surrogate epigenetic biomarkers and identify methylation sites in GAP43, ATP2A3 and ADARB2 genes as potential targets in relation to overweight management and insulin sensibility.