Resumen de Rosemary Extract‐Mediated Lifespan Extension and Attenuated Oxidative Damage in Drosophila melanogasterFed on High‐Fat Diet
Hua‐li Wang, Zhen‐ou Sun, Rizwan‐ur Rehman, Hong Wang, Yi‐fei Wang, Hao Wang
Rosemary extract has a potent antioxidant activity and is widely used in the food industry. In this study, the lifespan prolonging and antioxidant activity of rosemary extract was evaluated by high‐fat‐induced oxidative damage in Drosophila melanogaster. The results revealed that the lifespan and climbing ability of fruit flies was enhanced significantly by feeding rosemary extract. Furthermore, feeding with rosemary extract significantly increased the enzyme activity of superoxide dismutase (SOD) and catalase (CAT), and significantly decreased the level of malonaldehyde. The gene expression of SOD, CAT, and nuclear factor erythroid‐2 related factor 2 was enhanced and that for methuselah was significantly reduced. The comet assay showed that high‐fat diet‐induced DNA lesion was significantly reduced in larvae treated with the rosemary extract. Our results suggest that feeding with rosemary extract is effective to the extended lifespan in fruit flies by strengthening of the resistance to high‐fat‐induced oxidative stress and by stimulating, at least in part, the endogenous antioxidant response. Rosemary extract is a potent antioxidant in nature. Despite extensive research on rosemary extract, little is known of how it interacts with the genes involved in delaying aging and prolonging the lifespan of a whole organism in vivo. Our present finding shows that rosemary extract extended the lifespan, attenuated the oxidative damage, and increased antioxidant enzymes activities in fruit flies, which is useful for rational utilization of rosemary extract in functional food.
