Extraction optimization, antioxidants, and lipid-lowering activities of polygonatum cyrtonema Hua polysaccharides

• Jin Zuo ^[1] ; Wei Liu ^[1] ; Lei Tian ^[1] ; Mengyan Wang ^[1] ; Chunyan Liu ^[1] ; Min Wang ^[2] ; Zheng Fang ^[1] ; Jun Han ^[1] ; Guodong Wang ^[1] ; Qin Yin ^[1]
  1. [1] Wannan Medical College

     Wannan Medical College

     China

     
  2. [2] Wuhu Medical and Health School
• Localización: CyTA: Journal of food, ISSN 1947-6337, ISSN-e 1947-6345, Vol. 22, Nº. 1, 2024
• Idioma: inglés
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• Resumen

  • Polygonatum cyrtonema Hua polysaccharides (PCP) have beneficial effects on lipid metabolism and oxidative stress. Here, PCP extraction was optimized using response surface methodology (RSM), and the antioxidant and lipid-lowering effects of its main component, PCP1, were evaluated in vitro. The maximum PCP yield was 11.05% at an extraction temperature was 72°C, liquid-to-solid ratio of 21, extraction time of 96 min, and two extraction rounds. PCP1 was purified by column chromatography and showed significant scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and 2, 2'-azino-bis 3-ethylbenzthiazo-line-6-sulfonic acid (ABTS) radicals at concentration of 0.2–1.0 mg/mL. At a concentration of 200 μ g/mL, PCP1 significantly improved lipid disorder and damage induced by palmitic acid in HepG2 cells. These results show that PCP has potent antioxidant and lipid-lowering effects in vitro; moreover, the PCP extraction rate is significantly increased using RSM. Therefore, PCP is an effective antioxidant and lipid-lowering product with potential medicinal applications.