Protective effects of Maillard products of skipjack trypsin hydrolysate on uric acid-induced injury of human kidney-2 cell (HK-2)

Yurou Chu ^[1] ; Jiaxing Wang ^[2] ; Jipeng Sun ^[2] ; Ru Song ^[1] ; Liang Chen ^[1] ; Xiaoyu Zou ^[1]
1. [1] Zhejiang Ocean University
  
  Zhejiang Ocean University
  
  China
2. [2] Zhejiang Marine Development Research Institute
Localización: CyTA: Journal of food, ISSN 1947-6337, ISSN-e 1947-6345, Vol. 22, Nº. 1, 2024
Idioma: inglés
Enlaces
- Texto completo
Resumen
- The uric acid (UA) induced damage influences human health and deserves attention. The purpose of this study was to investigate the protective effects of Skipjack trypsin hydrolysate (STH) and its Maillard product (STH-M) and the segmentation of STH-M (STH-M-S) against UA-induced HK-2 cells. The CCK8 assay was used to detect cell viability and flow cytometry was performed to check cell apoptosis and cell cycle and the functional mechanism was analyzed via transcriptome. The results showed STH-M-S could significantly improve the cell viability and inhibit cell apoptosis. Meanwhile, STH-M-S could influence the membrane and protein binding and regulate metabolic-related and immune-related pathways to deal with UA stress. Functional genes contained NEBL, KCNJ16, ABCA12, CLDN2, FLRT3, PRODH, KLHL14, VEPH1, HPGD (down regulated) and FOXL1, SCG5 (up regulated). Collectively, our results suggested that STH-M-S can alleviate cell injury induced by UA. STH-M-S could be an alternative to be applied for the treatment of hyperuricemia.